The advancement of technology in the field of 3D printing of metal alloys allows for creating very complex structures. Newfound technologies of manufacturing, however, require testing of basic mechanical properties of the new structures. This paper is dedicated to the analysis of mechanical properties of a trabecular Ti-6Al-4V titanium alloy. Due to its complex morphology, the trabecular structure could not be created by traditional manufacturing process and was made by 3D printing. It was found that by creating this structure, the Young’s modulus of the whole body of the specimen was significantly reduced. The methods used for the determination of the mechanical properties are nanoindentation, tensile and compression tests.