This contribution describes analytical evaluation and experimental verification of the friction and force conditions in the pre-stressed bolted connections. In the case of using standard fasteners (bolts and nuts), there were evaluated mainly influence of advanced coatings using and lubricants and modern insurance sealants and adhesives into the threaded surfaces of bolted joints. To achieve the set goals, modular design-testing stand was designed. Achieved analytical findings and experimental verification will allow to refine the technological processes of assembly of bolted joints, which will have a major impact on the strength, durability, and reliability of the screw connections (fasteners). Discovered knowledge is not currently available for design engineers during the designing of bolted joints. Design engineers typically use only recommendation of retailers and manufacturers when designing of bolted joints. In industrial practice, it is now very often required to calculate the screws according to the standard VDI 2230. Standard VDI 2230 specifies a procedure for the strength calculation of pre-stressed screw connections. The calculation is usually done on a personal computer using the KissSoft program.