Article deals with describing of the pre-testing procedure for the uniaxial testing, nanoindentation and finite element modeling of single trabeculae. The first part is focused on the specimen resection, delipidation and fixation in a novel high precision experimental device which enables to measure the strains optically using a high-resolution CCD camera. The second part deals with the development of the volumetric model of the trabecula required for numerical analysis. The last part is dedicated to optimization of polishing procedure used sample surface preparation for nanoindentation. Effect of the grain size, load and duration time of the grinding on final surface roughness was analyzed. Change in the surface roughness was monitored during the preparation process using a confocal microscope. Optimized procedure of sample preparation for micro-mechanical testing with satisfactory results is presented in the paper.
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